Composite

Part:BBa_K5186007:Design

Designed by: Jiawen Chen   Group: iGEM24_AIS-China   (2024-09-25)


PTac-riboJ-DXS-IspG-DXR-B0015


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3332
    Illegal XbaI site found at 132
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3332
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3332
    Illegal BglII site found at 2474
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3332
    Illegal XbaI site found at 132
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3332
    Illegal XbaI site found at 132
    Illegal AgeI site found at 1392
    Illegal AgeI site found at 3631
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 886
    Illegal SapI.rc site found at 2388



Source

DXS(BBa_K3166061), IspG(BBa_K1653001) and DXR(BBa_K1950003) are from E. coli

References